Ultra performance liquid chromatography (UPLCTM) takes advantage of small 1.7 µm particles operated at elevated pressures to achieve uncompromised separation speed, resolution, and sensitivity (1-5). In order to takle advantage of this new technology, it will be necessary to migrate methods from high performance liquid chromatography (HPLC) to UPLC, which can at first appear challenging. However, by selecting the proper column chemistry and dimensions, and properly scaling injection volume, flow rate and gradient conditions (if appropriate), the task of migrating methods is really quite easy and straightforward.
For Further Information you can download the following power point presentation.
http://rapidshare.com/files/195707699/HPLC_to__UPLC.rar
